Workshop Proposal:
Tuesday, 23

Title:

Advances in cell culture techniques for chromaffin cells and related cell types (PC12 cells and enterochromaffin cells).

Chair(s):

Livett, Bruce G.  (Melbourne, Australia)

Speaker 1:

Livett, Bruce (Melbourne, Australia) and Borges, Ricardo (Tenerife, Spain)
"Advances in cell culture for chromaffin cell and related cell types".

Speaker 2

López, Manuela G (Madrid, Spain)
"Experience in culture of chromaffin cells from human, mice and rat".

Speaker 3

Trifaró, José-María (Ottawa, ON, Canada)
"Labelling catecholamine stores in chromaffin cells in culture".

Speaker 4

Sørensen, Jakob (Gottingen, Germany)
"A strategy for structure-function studies of exocytotic proteins using chromaffin cells from knockout mice and viral rescue techniques".

Speaker 5

Gilabert, Juan Antonio (Madrid, Spain)
"Necessary conditions to maintain rat adrenal chromaffin cells in primary culture".

Speaker 6

Tischler, Arthur (Boston, USA)
"Culturing pheochromocytoma cells".

Speaker 7

Prinz, Christian (Munich, Germany)
"The art of culturing enterochromaffin cells"

Short description:
Studies making use of chromaffin cells, PC12 cells and enterochromaffin cell cultures  continue to contribute significantly to our knowledge about basic mechanisms of neurotransmitter release and action. These cells are derived embryologically from the neural crest and serve as a convenient source of neural cells (paraneurons) for neurobiologists interested in exploring molecular and cellular mechanisms of neuronal processes including neuronal development and signal transduction. Technical aspects of the isolation and culture of these cells are often lost in the fine print of Methods sections of published papers. Nevertheless this information is essential to anyone practicing the art of cell cultures of these and related neuronal cells (including PC 12 cells and enterochromaffin cells). Protocols have been refined in different labs but not officially published. Primary monolayer cultures of chromaffin cells and PC 12 cells and suspension cultures of these and related cell types demand different protocols: specialized enzymes and plasticware, growth factors and optimized culture media.  It is now timely to review and update the information gained by hundreds of researchers who have used these cultures and related cell culture systems. This workshop will address these important technical issues.

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